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文献和实验) Stains‐all (Aldrich) 4‐ and 8‐mL screw‐thread glass vials (Wheaton) 15
Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
of supercoiled DNA) you may wish to plate two dilutions of each to ensure isolated colonies. Take 10 μl and 100 μl of each culture and plate on LBM Amp plates (if ampicillin is the selection) using a glass spreader. The 100 μl plates should be quite dense. Flame
would yield sufficient insert containing clones, independent of the repair process. Protocol 1. Combine the following reagents in a microcentrifuge tube, and incubate overnight at 4℃: DNA fragments 100-1000 ng cloning vector 2 μl (10 ng/μl) 10X ligation
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