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上海希言科学仪器有限公司
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文献和实验, 70% ethanol Formamide (FA), (e.g. from Merck) Master mix ( 20% dextransulfate/4xSSC) SSC (20x) 70% FA/2xSSC for chromosome denaturation (120 µl per slide) 18x18 mm2 cover slips, 60x24 mm2 cover slips (e.g. Menzel) rubber cement (Fixogum
Spotted Microarray Hybridization and Scan
Carefully transfer the probe solution to a new tube avoiding any pelleted material. Carefully pipette the hybridization mix onto the array (between marks etched on back of array) Overlay with a clean glass cover slip
or 3% hydrogen peroxide for 10 min at room temperature. Cover the pan during the soak.4. Prep 5000 mL 1x TAE5 for the Hoeffer unit or 3300 mL 1x TAE5 for the BioRad unit. Pre-chill the buffer on ice in the coldroom.5. Cut nylon
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