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文献和实验Subculture of Suspension Cell Lines
are known to adhere strongly. Pipette trypsin/EDTA (Prod. No. T4049) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask to cover the monolayer with trypsin. Decant the excess trypsin. Return flask to the incubator and leave
Alternative Cell Culture Systems
and incubated under the culture conditions appropriate for the cell line. Cultures should be stirred at 100-250 revolutions per minute. Spinner flask systems designed to handle culture volumes of 1-12 liters are available from Techne, Sigma, and Bellco, e.g
Subculture of Suspension Cell Lines
. Pipette trypsin/EDTA (Prod. No. T4049 ) onto the washed cell monolayer using 1ml per 25cm2 of surface area. Rotate flask to cover the monolayer with trypsin. Decant the excess trypsin. Return flask to the incubator and leave for 2-10 minutes
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