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文献和实验seeds c. Place a 4 mm stainless steel grinding ball in each well of the 2 ml 96 square well block and cover with sealing mat. Place block in the bead mill and shake at 1,500 rpm for 10 min. d. Continue to Extract seeds DNA.
1, For cells cultured in 2D, about 1-2X107 S1 cells were growth in 100mm dish, and were cross-linked by adding formaldehyde to final concentration of 1% and incubated in room temperature for 10 minutes. For cells growth in 3D, cells
【转帖】2-D Clean-Up Kit 双向凝胶电泳--蛋白纯化
in the 1st dimension. The 2D Clean-Up Kit from GE Healthcare (formerly Amersham Biosciences) is the most effective method of getting rid of such impurities and thus, sample preparation with this kit results in proper isoelectric focusing and minimal streaking
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