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文献和实验that hole with a cap QS-T from ISOLAB Inc. (Drawer 4350 Akron, OH 44303, 100 caps for $ 9.50). 2. The other problem that may occur is that the nebulizer leaks where the hose for the nitrogen gets attached. It seems that Nalgene tubing (VI grade 3/16" ID
that hole with a cap QS-T from ISOLAB Inc. (Drawer 4350 Akron, OH 44303, 100 caps for $ 9.50). 2. The other problem that may occur is that the nebulizer leaks where the hose for the nitrogen gets attached. It seems that Nalgene tubing (VI grade 3/16" ID
, harvest 24-48 hrs post transfection. LipofectAMINE Transfection of NIH3T3 Cells 1. Plate 3T3 cells at 2 x 105 per 35 mm dish & let grow overnight (cells should be 50-70% confluent for transfection). 2. For each transfection, dilute: A: 8 μl
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