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文献和实验Desalting Oligonucleotides by Gel Filtration
tray underneath the column to catch the waste. Remove the top off the NAP-10 column and empty the storage liquid into the drip tray. CAUTION: Avoid contact with the storage liquid. This solution may contain poisonous preservatives such as Kathon
Southern Blotting: Hybridization, Washes, and Development
by washing 2 x 15' with Buffer 1. 8. Equilibrate blot with Buffer 3. C. Visualization 1. Prewarm CSPD Ready-to-Use to RT. 2. Transfer blot carefully from Buffer 3 (let drip ~5'') to a plastic tray. 3. In the darkroom, incubate
the excess EtOH to drip off (can touch to a Kimwipe to remove but don't let it over dry) and immerse in 1 ml of TE, pH 8 overnight at 4℃.Free the DNA by gently scraping with fresh sealed pipets.Remove the pipets and rotate at 4℃ (24-48 hrs or longer
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