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文献和实验Worm PCR Pick one worm and place it in a 2.5 l drop of lysis buffer in the cap of a PCR tube. Close and centrifuge briefly to move to the bottom of the tube. Freeze the tubes at -70°C for 15 min. The idea is to do a freeze
ICAM-1纯化 Purification of ICAM-1
ml of Elution Buffer. Elute 3ml in each prepared collection tube. Mix each tube immediately after elution and cap with parafilm to prevent evaporation during storage. Strip the column with 5 column bed volumes of Stripping Buffer. Equilibrate
NaCl 4.4g dH2 O 450ml Adjust pH to 8.0 using HCL. Add water to 500ml final volume. Add 1g Octyl Glucoside to 100ml of Wash Buffer. Add 1ml freshly prepared 100mM PMSF to each 100ml Wash Buffer. Final concentration of 1mM. Add
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