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文献和实验Cotton Pistil Drip Transformation Method
could efficiently generate more transgenic plants and allow meaningful analyses of gene function and transgenic plants. Cotton pistil drip by inoculating Agrobacterium tumefaciens onto the pistil after pollination gave rise to stable transformants
Membrane Transfer and Crosslinking for RNA
Since different types of gels are used for these techniques, the mode of transfer is different in each case. Agarose gels are used for Northerns because of their wide range of resolving power and large loading capacity. Their porosity allows efficient passive
Arabidopsis transformation by floral dipping（拟南芥转化）
bottles. Spin down the cells (12 min in Jouan benchtop centrifuge, 3000 rpm). As soon as the centrifuge has stopped, open the bottles and decant the supernatant back into the Erlenmeyer flask. The supernatant has to be treated as bacterial waste (autoclave
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