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上海希言科学仪器有限公司
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文献和实验Homogenizer, RNase-free syringe (1 mL) with 18–21-gauge needle or, Rotor-stator homogenizer Microcentrifuge capable of centrifuging 12,000 × g 15 mL RNase-free tubes (for samples with >107 cells) 实验步骤 1.
of tissue/ cell harvest. Dynabeads® mRNA DIRECT™ Kit protocols can be scaled up or down to suit specific sample source and quantity. Please see section "Sample Guidelines and Scaling" before preparing the sample, and for recommended bead and buffer volumes
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
Embryonic Fibroblasts (Irradiated) 4. DMEM with GlutaMAX™-I (High Glucose) 5. KnockOut™ DMEM/F-12 6. Fetal Bovine Serum (FBS), ES Cell-Qualified 7. KnockOut™ Serum Replacement (KSR) 8. MEM Non-Essential Amino Acids (NEAA)
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