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文献和实验Ice· Micropipettes,0.5 to 1.0 µl,10 to 100 µl,100 to 1000 µl·Sterile pipette tips·Disposable gloves,safety glasses,and lab coats·Microcentrifuge,for 1.5 ml and 0.2 ml PCR (adapter)microcentrifuge tubes·Sterile deionized water·Template DNA·Primers
RNASafer® Protocol for Total RNA Isolation
buffer and mix with 350ul 70% ethanol. For RNA isolation using Midi and Maxiprep kit, follow the standard protocol in the user manual. 2) Apply the sample to the HiBind® RNA column and follow standard protocol in user manual for each kits
Streptomyces:Protocols/Mini-Maxi Prep
in which the work is done. All users need to understand the risks associated with both phenol & chloroform. A phenol decontamination kit (PEG300:Meths) must be located near the fume hood in which the work is done. General laboratory & molecular microbiology safety
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