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文献和实验and measure protein concentration (BioRad assay) use 1 - 5 mg protein per IP immunoprecipitate for 2 h to ON, 4 °C wash immunoprecipitations pelleting the beads each time: 2 x 1 ml CHIP lysis buffer 2 x 1 ml CHIP
_ChIP_method.html Chromatin IP Method, ChIP buffers, Notes on ChIP Method, Quantative PCR using 32P dCTP. Hahn Lab. ChIP Assay Protocol PDF - /attach/2008/2008-02-01/2B/2B6C4EA25F257424710A50E83CB2F380.pdf Formaldehyde cross-linking
Chromatin Immunoprecipitation (ChIPs) Cloning Protocol (Farnham Lab)
among your samples. If starting with 1 x 108 cells, then divide into 9 samples; 8 to be precipitated with the antibody used for cloning and 1 for a no antibody or preimmune serum control. We also include a "mock" sample which contains IP dilution buffer
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