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文献和实验36 x 0.2ml Standard Micro-tubes 72 x 0.1ml Strip tubes Heating/Cooling Rate: 2.5°C/second (tube temperature) 5.0°C/second (air temperature
CGH of Biotin Labeled DNA vs Digoxigenin Labeled DNA
ul of 4X SSC/1% BSA). Gently cover with a 24 X 50 mm coverslip. Incubate for 45-60 mins (protecting from light). Gently remove coverslip. Wash 10 mins each at RT in 4X SSC, then 4X SSC/0.1%Triton, then 4X SSC. Rinse slides 2X
Wash slides 3 x 3 min at 37°C in FA / 2x SSC (new cuvette). Wash slides 3 x 2 min at 60°C in 0.1x SSC (new cuvette). Keep slides in 4x SSC / 0.1% Tween20 at 37°C (new cuvette) until the next step. Cover each slide with 125 µl 3% BSA
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