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上海希言科学仪器有限公司
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文献和实验and neutralizing solution. Tray with neutralizing solution. Whatman #1 filter paper to layer between filters. 80℃ vacuum drying oven (preheated 1 hour). Hybridization buffer (sterile 5x SSPE; 10x filter sterilized Denhardt's solution; sterile 0.1% SDS; use
two hours. Add 350 m l Stop Solution. Incubate at 37°C for 30 min. (optional). Add 350 m l PCI-9, mix by vortexing, spin in microfuge 30s, transfer the supernatant to a new tube and repeat xl (2 PCI extractions total). Add 350 m l
in the RC5-B using the SS-34 rotor. Gently decant the supernatant, add 80% ethanol, centrifuge as before, decant, and dry the DNA pellet in a vacuum oven. 15a. Resuspend the DNA in 10:0.1 TE buffer. For diatomaceous earth-based purification:
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