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文献和实验Cell-Free Ion-Channel Recording
from the underlying cytoskeleton, one irreversibly changes the integrated system of which the channel protein is a part, and begins an inexorable loss of channel function. In this chapter we will deal primarily with the techniques available for measuring the response
Cell Migration in Confinement: A Micro-Channel-Based Assay
This chapter describes a method to study cells migrating in micro-channels, a confining environment of well-defined geometry. This assay is a complement to more complex 3D migration systems and provides several advantages
Patch-clamp is the best method for analyzing single-channel current activities today. The method is not good, however, at measuring the total number of channels expressed on the cell membrane that are responsible for the current of concern
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