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文献和实验In situ immunodetection of 5-methylc
8) Wash in PBST 2x5min 9) Incubate slides in streptavidin Cy3 (1:300 dilution in PBST) for 1h at 37℃ in a humid chamber 10) Wash in PBST 2x5min 11) DAPI stain and mount (Protocol 9.3 in situ book) Solutions: PBS (see Appendix 1 in situ book) PBST
In situ immunodetection of 5-methylcytosine (5-mC) on squashed cells using anti-methylcytosine
in PBST) for 1h at 37oC in a humid chamber 8) Wash in PBST 2x5min 9) Incubate slides in streptavidin Cy3 (1:300 dilution in PBST) for 1h at 37oC in a humid chamber 10) Wash in PBST 2x5min 11) DAPI stain and mount (Protocol 9.3 in situ book)
免疫组化技术-Immunohistochemical Protocols
into the subbing solution, drain the slides onto a paper towel and allow to air dry for 1 hr. Dip the slides into the subbing solution again. Drain and cover loosely with plastic wrap or bench paper.
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