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文献和实验from the reservoir). 5) Sterilize the above parts by steam autoclaving at 121 °C for 60 min. Additionally autoclave one complete flow chamber, 3 x 2" segments of soft tubing, 1 male and 1 female 1/8" luer adaptor, 4 x ½" and 6 x 5/16" 8-32 flat head
UPDATED DIRECTIONS FOR NEW BRUNSWICK MOBILE PILOT PLANT FERMENTOR
cap on headplate and add media to desired level. 10 L min, 30 L max. Add antifoam. (Do not use a silicone based antifoam if the tangential flow concentrator will be used!. Use Antifoam 204; ~1/4 capful/30 liters). Replace filling port cap. 2. Set
to pH 8.5 (test with pH strips, not with pH meter to avoid introducing RNA ses).Then add: 1.5% w/v N-lauroylsarcosine (Na salt) 1.50 g 1.0% NP-40 (nonidet P-40) 1 ml 1.0% sodium deoxycholic acid 1.00
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