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文献和实验of 0.5,1,2μmol/L, respectivel y) for 4 successive days. The cell growth and proliferation were observed by cell counting and cell-growth curve. Morphologic changes were studied with electronmicroscopy. Flow cytometry was used to assay cell-DNA distribution
Marcantonio Lab Protocol Manual
of histidine solution (21 mg/ ml) 10 ml sodium pyruvate solution (100x) (GIBCO) 10 ml Penicillin/Streptomycin Solution (GIBCO) 5) Adjust the pH to 7.00 with HCl, using the pH meter. 6) Dilute the medium to the appropriate volume using
STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
. No. Bg100) contains rtagents sufficient for 200 tests. GUS- Light (Cat. No. BG30O) contains reagents sufficient for 600 tests. Glucuron Chemiluminescent Substrate A l00X concentrate is diluted in GUS Reaction Buffer Diluent prior to use. GUS Lysis
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