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文献和实验or cosmid DNA of interest into a 12 X 75 mm Falcon tube containing 2 ml of LB media supplemented with the appropriate antibiotic (typically ampicillin at 100 ug/ml) and incubate at 37deg C 8-10 hours with shaking at 250 rpm. Transfer the culture
S1 Nuclease Protection Assay End-label oligo (20-25 mer)4 µl 5X T4 Kinase Buffer5 µl [gamma-32P] ATP (7000 Ci/mmol)10 µl water + oligo (200 ng)1 µl T4 Kinase1. 37 deg C for 1 hour.2. Heat inactivate at 75 deg C for 10 minutes.3. Add 1 µl glycogen
【交流】染色体免疫沉淀技术详细步骤-young lab !!!
supernatant and resuspend pellet in 10 ml 1x PBS per 108 cells. 6. Transfer 5x107 – 1x108 cells to 15ml conical tube and spin at 1,350 x g for 5 minutes at 4°C (Sorvall Legend RT centrifuge with swinging bucket rotor). Discard supernatant. 7. Flash freeze
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