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文献和实验线粒体荧光探针大全:TMRM,Mitotracker,JC-1
and of the verotoxin B subunit in Vero cells 30 Our Vybrant Apoptosis Assay Kit #11 (V35116, Section 15.5) utilizes MitoTracker CMXRos in combination with Alexa Fluor 488 annexin V in a two-color assay of apoptotic cells (Figure 15.95). MitoTracker Orange CMTMRos
) Nuclear lysis buffer Oligonucleotide linkers (optional; see Step 18) PBS (phosphate-buffered saline [pH 7.4]) PCR product purification kit (e.g., QiaQuick PCR purification kit, QIAGEN) PCR reagents (e.g., Taq polymerase
. Electropherogram of Amplified aRNA Sample. Total RNA 2 µg corresponding to 60 ng mRNA) was amplified using the MessageAmp aRNA Kit (Ambion Cat# 1750) resulting in (90 µg aRNA, a 1500 fold amplification. The aRNA (900 ng) was analyzed on an Agilent 2100 bioanalyzer
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