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文献和实验Fluorescence-Based On-Line Detection as an Analytical Tool in RNA Electrophoresis
In this chapter, we present methods for denaturing and native gel electrophoresis of nucleic acids based on fluorescently labeled probes and automatic signal detection by a deoxyribonucleic acid sequencer. Specific examples are given
Sample preparation (analytical gels)
. ELC (erythroleukemia cell line): A monolayer culture of human ELC was grown, rinsed trypsinized and washed as explained in the HEPG2 sample preparation. A pellet of 0.8 x 106 cells were mixed and solubilized with 60 μl of a solution containing urea
Analytical Centrifugation: Equilibrium Approach
representation of an analytical ultracentrifuge. The rotor (C ) has holes through it to hold sample containers commonly called cells (D ). Each cell (see inset) consists of a centerpiece (G ) with open‐sided chambers called channels (H ) to hold the liquid
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