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文献和实验Analys of Genomic DNA by Southe
mM Tris pH 7.5. (Residual ethanol can cause the DNA sample to 'crawl' out of the well when loaded onto the gel. Heating the solution of redissolved DNA to 65-70℃ in an open tube for 10 minutes can usually drive off most of the ethanol.) 4.Add 3 µl 6 x
Single and Double FISH protocols for Drosophila
3. Following the transcription reaction (see Note #2), the labeled probe is precipitated by addition of 1 ul of 0.5M EDTA, 2.5 ul 4M LiCl, and 75 ul absolute ethanol. Chill to -70oC, spin and wash the pellet as described above. After drying, resuspend
Analys of Genomic DNA by Southe
to 'crawl' out of the well when loaded onto the gel. Heating the solution of redissolved DNA to 65-70℃ in an open tube for 10 minutes can usually drive off most of the ethanol.) 4.Add 3 µl 6 x DNA-loading buffer (total volume 18 µl). (6-16
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