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文献和实验Rapid DNA Amplification Using a Battery-Powered Thin-Film Resistive Thermocycler
heater and a miniature fan enable rapid heating and cooling of glass capillaries leading to a simple, low-cost Thin-Film Resistive Thermocycler (TFRT). Computer-based pulse width modulation control yields heating rates of 6–7 K/s and cooling rates of 5 K
.6. Heat the samples at 90C for 5 min, then cool on ice. While the samples are cooling, again rinse out the wells with buffer, being sure to rinse out all the urea-containing buffer from each well.7. Using a drawn-out glass capillary & mouth pipetter, load
Microwave Processing Techniques for Microscopy
of 30 seconds is required to operate at half its power (350 W), the magnetron is on for the first 15 seconds and off for the subsequent 15 seconds. In laboratory use, this often results in a cycle of heating and cooling that produces suboptimal
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