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axis of the cord and had also begun to migrate rostro caudally from injection site. They were visualised here using the protocol followed by a TRITC conjugated secondary antibody Scale = 500µm Fig. 3 BrdU In vivo cells were labelled
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
medium for OP9 cells (for Protocols 1, 2) Leukemia inhibitory factor (LIF; 10 µg/mL; Chemicon LIF2010) (for Protocol 2; see Step 20) Lympholyte-M (Cedarlane CL5120) (for Protocol 3) Mice, 4-8 wk old (for Protocol 4; see Step 69) Mice, fetal
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