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文献和实验DIRECT AND SHORT-TERM PROCEDURE FOR HARVESTING BONE MARROW CHROMOSOMES
into a 15 ml screw-cap conical centrifuge tube; transfer the tape label making sure no label mistakes occur. Centrifuge the tubes for 6 minutes at 150 x g (900 rpm). D. Aspirate and discard all but 0.5 ml of supernatant, make sure to remove the fat
& top, remove the filter from its packaging and screw it onto the bottle. The filter is sterile, so try not to touch it to anything else Attach the adapter to the filter and the vacuum nozzle to the adapter (vacuum should be off) Pour the CSM
to the wet wt divided by 0.5 g/ml minus wet wt. Divide the S med to each of the six bottles, cap well Shake in secured box for about 1 hr at 250 rpm at RT Measure volume of one bottle, record and transfer contents of other five bottles
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