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文献和实验Lipid analysis-Week 3: GAS LIQUID CHROMATOGRAPHY
it to the attention of the instructor or technician. C: Sample injection 1. Use the 10.0 µl syringe provided and labeled for the GLC only, do not use any other syringe for your samples. Rinse the syringe out with hexane provided
Preparation and purification of biotin-labeled probe for ISH
; and electrophorese the sample on a mini-gel with a DNA size marker. The size of the nick translation product can be adjusted by adding a different amount of DNase I in the reaction solution. Random primer labeling also can be used for biotin-labeling
, and 20 mMTris-HCl, pH 7.5, sterile filtered. 5. Syringe (30–50 mL) with 0.2-μm pore size filter. 2.3.2. Step 2: IMAC 1. Sample: Pooled Wnt3A containing fractions from Section2.3.1 . 2. HiTrap? Chelating, 1-mL column (GE Healthcare, Piscataway
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