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文献和实验Maintenance of Probes in bacteria including Escherichia coli
agar. Use airtight, autoclaved vials containing 2/3 full (appr. 1 ml) of medium. Inoculate each vial with a single colony. Leave the cap of the vial sightly loose and incubate overnight at 37 C.
Maintenance of Probes in bacteria including Escherichia coli
in glycerol solution at -20 C. At -70 C they can be stored almost for life time. Prepare 0.5 ml of glycerol solution in 2.0 ml screw-cap vials, autoclave (15 min, 121 C), cool, and store them at room temperature.
Affinity Purification Of Antibodies
ON in 0.1X PBS, A280, pool peak and lyophilize.(8) Check peptide coupling (1mg samples) on a Laemmli gel.2. Affinity Column Prep(1) Affigel 102 (BIORAD) washed in PBS on a scintered glass funnel (3ml/column).(2) Transfer to 15ml screw cap tube in minimal
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