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文献和实验Lipoprotein Isolation - First week
ml plastic syringe (held by a clamp) attached to a 18 gauge needle and a capillary tubing (see picture ). 10. Let the hemolymph flow into the heat sealable centrifuge tube. 11. Add another 20 ml PBS into the syringe and overlay
Lipoprotein Isolation - First week
needle and a capillary tubing (see picture ). 10. Let the hemolymph flow into the heat sealable centrifuge tube. 11. Add another 20 ml PBS into the syringe and overlay on the hemolymph. 12. Turn on the heat sealer to warm it up. 13
: cold9. chloroform/butanol: 4:lThe following is protocol for 1.6 g of needle or phloem tissue, which is a suitable volume for one 50-ml orange cap tube (Corning, conical) that you will need for processing. Scale up by nX the following protocol:Chill
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