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文献和实验In Situ Hybridization Using Digoxigenin Labeled Probes
to the drop of fixative. 7) Place the slide, coverslip up, on several sheets of paper towel. With another sheet of paper towel hold the edge of the coverslip to prevent it from moving. 8) Using a stiff, pointed probe, gently press once over the glands
Screening BAC filters with non-radioactive probes
Science, Owashi 1-2, Tsukuba, Ibaraki 305-8634 , JAPAN) Probe labeling ( ECL Direct Nucleic Acid Labeling & Detection System. Amersham Biosciences Catalog #RPN3001 Take 500 ng DNA and 0.25 ng pBR322 in a Eppendorf tube
, save foil Remove foil and attach air in to the back port and air out to the front, save foil Plug in temperature probe on right side and place into temp port, fill with ddH2 0 Place digital temp probe (borrow from Ken) into temp port
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