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文献和实验PipMaker: A World Wide Web Server for Genomic Sequence Alignments
, J., Gibbs, R., Hardison, R., and Miller, W. 2000. PipMaker: A web server for aligning two genomic DNA sequences. Genome Res. 10:577‐586 Waterston, R., Lindblad‐Toh, K
Generation of HPV Pseudovirions Using Transfection and Their Use in Neutralization Assays
) can also be produced after transfection of 293TT cells with L1 alone. Efficient purification of the PsV or VLPs is achieved by Optiprep (iodixanol) density gradient ultracentrifugation. Using these methods, it is possible to produce highly purified PsV with yields
Using MicroRNAs to Enhance the Generation of Induced Pluripotent Stem Cells
50‐ml conical tubes (Costar, cat. no. 430828) 75‐cm2 flasks (BD Falcon, cat. no. 353136) 1.7‐ml
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