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文献和实验Cigarette Smoke Exposure as a Model of Inflammation Associated with COPD
) Rubber bungs/stoppers, 30‐mm (VWR International) Masterflex silicone tubing L/S 15 (Cole‐Parmer) PVC
Embryo Manipulation Pipettes (Flushers etc.)
stand clamps, set the tubing up so that it is stable with the needle pointing upwards. Place the adjustable screw clamp around the tubing (below the retort stand clamps) and tighten fully. Connect the other end of the tubing to a gas outlet
all material to the temperature at which the chromatography will be performed. 2. De-gas the medium slurry. 3. Eliminte air from the column dead spaces by flushing the end pieces with buffer. Make sure no air has been trapped under the column
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