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文献和实验Lee, S.H., Lumelsky, N., Studer, L., Auerbach, J.M., and McKay, R.D. 2000. Efficient generation of midbrain and hindbrain neurons from mouse embryonic stem cells. Nat. Biotechnol. 18:675‐679
): is used in the 1st Dimension (Righetti, P.G., 1983). This separates proteins by their charge (pI).Isoelctric focusing (IEF) can be described as electrophoresis in a pH gradient set up between a cathode and anode with the cathode at a higher pH
in the 1st Dimension (Righetti, P.G., 1983). This separates proteins by their charge (pI).Isoelctric focusing (IEF) can be described as electrophoresis in a pH gradient set up between a cathode and anode with the cathode at a higher pH than the anode
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