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文献和实验has been solved in some cases by lowering the concentration of the ß-gal DNA construct injected. 3. Setting up the scope, loading the needle, mounting, and breaking the needle. Set up the scope and inector: We use a Zeiss Axiovert 10
slots from the underside in order to penetrate to the reservoir on the inflow and outflow ends. 4) At the end of each top plate, drill a centrally located 10/32" tracks per inch (T.P.I.) hole for polypropylene 1/8" hose barb to penetrate
Single-molecule analysis of DNA replication by molecular combing
from the reservoir at the speed of 300 µm/s (see note 4). Repeat with another coverslip as many times as needed. Check DNA fibers under the microscope using a 40x objective and a FITC filter block. To this aim, attach the coverslip to a metal holder with a hole
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