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文献和实验Pre-Cast Gels for Low-Throughput Nucleic Acid Analysis
(with the comb in place) into the apparatus right edge first. Press firmly at the top and bottom to seat the gel in the base. You should hear a snap when it is in place. The Invitrogen logo should be located at the bottom of the base, close to the positive pole
IN SITU HYBRIDIZATION ON FROZEN SECTIONS
into the bubble covering the section. Incubate overnight at 55°C. (Adjust volume if you have used less than 100µl for prehybridization). Wash 2 times 10 min. each in 2x SSC with betaMercaptoEtOH-EDTA, RT. (discard to radioactive WASTE) Immerse in RNase
IN SITU HYBRIDIZATION ON FROZEN SECTIONS
to radioactive WASTE) Wash 2 hours in 4 liters of 0.1x SSC with betaMercaptoEtOH-EDTA , 55°C. Wash 2 times 10 min. each in 0.5x SSC without betaMercaptoEtOH or EDTA, RT. Dehydrate 2 min. each in 50%, 70%, and 90% ethanol containing 0.3M NH4Ac
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