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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
is off. 2. With Charge/Pulse switch of the electroporator in the PULSE position, connect the leads from the power supply to the corresponding coloured terminals on the back of the electroporator. 3. Set Arm/Disarm dial to disarmed 4. Set Capacitance
Lipid analysis－Week 3: GAS LIQUID CHROMATOGRAPHY
), computer is set to go and the GLC is in "run" mode, place the needle into the injector port. Port A located on top of the machine to the front left of center. The needle can be difficult to inject through the rubber septum as there is pressure
Injection of dsRNA into Drosophila Embryos for RNA Interference (RNAi)
capillaries into needles: i. Set heat at 12.5-13.0 and set the solenoid at 4 on the needle puller. ii. Follow the manufacturer’s instructions for pulling the needles. iii. Store freshly-pulled needles
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