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文献和实验Sleeping Beauty (SB) transposon-based transfection is a two-component system consisting of a transposase and a transposon containing inverted repeat/direct repeat (IR/DR) sequences that result in precise integration into a TA dinucleotide. The transposon
, or by adsorption to glass as in the commercial Qiex kit, and this will increase the efficiency of labelling considerably. - Boil the DNA to denature it for a few minutes. Briefly spin the tube and put on ice to cool. Add 6.6 µl 5X OLB, 5 µl hot dATP (50 µCi
the blot 3X30' in the 65° water bath. At this point the geiger counter should not detect counts on the blot if you are just probing for single copy genomic sequences. When probing with TC1 or other repetitive sequence probes, the signal may be hot
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