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文献和实验Use of Channelrhodopsin for Activation of CNS Neurons
Heat‐cure epoxy (e.g., blue dye epoxy; Fiber Instrument Sales) 3‐ml luer‐lock syringe with blunt 22‐G needle
Denaturing Urea-Polyacrylamide Gel Electrophoresis (PAGE) Based Microsatellite Analysis
. Use a needle attached to the syringe filled with 1x-TBE buffer to flush out all the wells. Denature PCR products (5 μl) along with 10-bp ladder mixed in 2X loading dye (20 mM EDTA, 0.05% Xylene cyanole, prepared in 95%formamide
V294.PART II,Chapter 4 细胞迁移实验之Dunn趋化迁移培养皿法及时序显微镜分析法
slip in place. The chamber consistsof two concentric circles ground into one face of a glass slide (inner and outerwells). An annular ridge (bridge) separates the two wells. Insert, the bridge is 20 μmlower than the surrounding glass slide resulting
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