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文献和实验. To transfer in a Biorad transfer cell: Fill the box ~3/4 with transfer buffer. Wet the two brillo pad-like stuffers with transfer buffer. Place one wet stuffer on the black half of the plastic holder, then pick up the glass plate carrying your sandwich, place
into hybridisation chamber. Add 10 of MilliQ water into the wells at each end of the chamber. Load cooled sample onto array slide and avoid bubles. Carefully place coverslip onto slide with fine forceps. Close and seal lid
Tissue Culture - Storage of Cell Lines
and resuspend in 10% DMSO/90% FCS at about 1-5 x 106 cells/ml. The freezing medium should be 5℃, not warm. 6.Put 1ml of cell suspension into cryovials and tighten the lid finger tight. Do not over tighten or the vials could explode when thawed. 7.Use the Nunc
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