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文献和实验Reverse Transcriptase-PCR Analysis of Gene Expression in Hematopoietic Stem Cells
with anti c-kit MAb and the lineage-negative cells from human bone marrow are incubated with anti-CD34 and anti-CD38 MAb. With a starting population of 2–4 � 10e8 unfractionated mouse bone-marrow cells, a typical isolation procedure yields approx 2 � 10e4 FR
High‐Throughput, Multiplexed Analysis of 3‐Nitrotyrosine in Individual Proteins
PBS‐T: 0.05% (v/v) Tween‐20 (Sigma, cat. no. P7949) in PBS Desiccant Plasma samples for analysis
per condition to allow enough cells for subsequent staining and analysis. Treat cell suspension with a titration of EOS9.1 mAb (a range of 1µg/ml to 0.06µg/ml is recommended). Cells in wells treated with medium only can be used as unstimulated cells.
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