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文献和实验Radioligand Binding Assays for the Glycine Site on N‐Methyl‐D‐Aspartate Receptors
GF/C filter mats (Brandel) or filter plates (Packard) Vacuum harvester (Brandel, or equivalent; unique harvester required for each tube format; LSM harvester for 48
.To minimize separation, wrap the gel in plastic film and store at 4°C until use. D: PCR Reaction TIP: Investigators must be especially careful when using this methodology to analyze archival tissue specimens. Formalin fixation in particular results in DNA
for 2hrs c) to digest w/ NcoI, add: 1ul Nco I (10U/ul) d) digest at 37C for 2 hours e) collect PMPs with magnetic stand, and remove the 40 ul of supernatent (Ligation template) to a new tube. The digestion should liberate the cDNA from the beads
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