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文献和实验microdissected cells in 20 µl proteinase K buffer.Incubate overnight at 37°C.B: Prepare the Glass PlatesTIP: Use Accuwipes for cleaning purposes, as they will not leave lint behind and are non-abrasive.Clean glass plates twice with glass cleaner.Repeat using 95
2-D Polyacrylamide Gel Electrophoresis
Bromophenol Blue as indicator. 6.Check volume is 50 ml. 7.Vortex until all is dissolved (or attach tube to a rotator). 8.Aliquot 1 ml into microfuge tubes. 9.Store at -20℃. B: 10X Electrophoresis Running Buffer (10 L)0.25 M Tris,1.92 M glycine,1M SDS
the dissecting hand, the dissector should prop their elbow on a solid surface adjacent to and at the same height as the stage of the microscope. It is helpful to rest the ulnar aspect of the dissecting hand on the stage of the microscope and move the needle
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