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文献和实验Hints and precautions for the care, feeding and breeding of Neurospora
number (about 200 per plate) can be obtained by adding or removing spores while examining the drop at 40-60 X magnification under the dissecting microscope. Spores are distributed evenly over the plate, using a sterile glass spreader. Do not spread
7.7 to a final concentration of 2.5 M and 2.5 volume of ice-cold 100% Ethanol to precipitate the DNA. 26. Centrifuge at 10,000 X g for 10 min at 4°C to pellet the DNA. 27. Wash the DNA pellet with 70% Ethanol. 28. Centrifuge at 10,000 X g for 10 min at 4°C
glass bottle CTAB Isolation Buffer (see Hint #5) 1.4 M NaCl 100 mM Tris-HCl, pH 8.0 20 mM EDTA 2% (w/v) Ethyltrimethylammonium Bromide (CTAB) 0.2% (v/v) 2-Mercaptoethanol 70% (v/v) Ethanol 7.5 M Ammonium Acetate, pH 7.7 TE pH 8.0 1 mM EDTA
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