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文献和实验Dynamic Monitoring of Cellular Remodeling Induced by the Transforming Growth Factor-β1
using 100 μl of complete cultivation media. BPH-1 cells were trypsinized, quantified, and seeded in additional 100 μl of cultivation media in a final concentration of 30,000 cells per cm2 . The cells were monitored continually every 1 min in the first 45
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
should be shake gently by rotating blood mixer (vortex) Pour 500 µl of blood into a 1.5 ml eppendorf tube and add 1000 µl of red cell lysis buffer. Shake microfuge tube gently (up to homogenizing), then spin for 2 minutes at 7000 rpm. Discard
Plasmid DNA Isolation from Bacteria
in a microcentrifuge. (observation: bacteria form a pellet at the bottom of the tube.) 2.Decant supernatant, leaving 50-100 μl in the tube. 3.Vortex to resuspend the bacteria pellet completely. 4.Add 300 μl of TENS solution. 5.Vortex for 5 seconds to mix
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