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文献和实验Expression Library Screening (Procaryotic) Using AP-Fusion Proteins
vector and can not be used for in vivo excision. Primers for gt11 are available from Sigma (forward primer: #P1680, reverse primer: #P1805). Growing up host bacteria Reagents: 1M MgSO4 (100 x
Construction and Characterization of Adenovirus Vectors
the tubes for 90 sec at 42°C and then for 2 min on ice. 7. To each tube, add 1 mL of LB. Incubate with shaking at 225 rpm for 25 min at 37°C. 8. Transfer the bacteria to 1.5-mL tubes. Centrifuge at 8500g for 1 min at room
Beta-galactosidase Reporter Gene Assay (Liquid Form)
to get different strains to reach OD600 nm ~ 0.8 at the same time. The best way to handle this is to start a 2 ml starter culture about 3-4 days before the assay. The night before the assay, start a 10 to 25 ml intermediate culture. The morning of the assay
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