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上海希言科学仪器有限公司
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文献和实验E-Z 96 X-press Plasmid Vacuum Protocol
selective antibiotic. Incubate 14-16 hours at 37℃ 600 with vigorous shaking until an OD of 2.0-4.0 is achieved. 2. Pellet 1-2 mL bacterial culture in a 2.0 ml 96-well deep-well plate by centrifugation at 3000 x g for 15 min at room temperature.
Genome-wide Screen for miRNA Targets Using the MISSION Target ID Library
Zeocin Invitrogen R250-01 Target ID Library Sigma-Aldrich MREH01
setting) and collect the mononuclear cell (MNC) (buffy coat) layer. Resuspend and wash MNCs x 3 with Dulbecco's Phosphate Buffered Saline (DPBS) with 10% Fetal Bovine Serum (FBS) x 10 min, 515 g, before plating into two 12-well plates in full EPC growth
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