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文献和实验Method for Single-Cell Electroporation
pulse induces sufficient transmembrane potential to disrupt the electrostatic forces maintaining lipid bilayer structure, causing the temporarily formation of small pores in the cell membrane. DNA and other charged molecules are then electrophoretically
Attach a 36" segment of hard tubing to one end of the pulse dampener (via 1/8" connection). To the other end, attach an 18" segment of soft tubing. 3) Place a 1/8" male luer adaptor at the end of the 18" soft tubing section, mentioned
PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of No. 30 G 1/2 needle is cut off and then rounded using sharpening stone); 1 ml syringe; Dissecting instruments (fine-pointed scissors, fine forceps); No. 5 forceps (Dumont); Mouth pipette (aspirator mouth piece, latex tubing, blue
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