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文献和实验HIGH RESOLUTION GENETIC FOOTPRINTING
stock solutions of Tris, EDTA, NaCl, Glycerol, and Hepes. Just before use, add DTT, reduced glutathione, and protease inhibitors where indicated, then pass through 0.2 um filter. 100 mM IPTG stock solution (MW=238.3) 595 mg IPTG 25 ml water
Procedures for In Vitro Production of Bovine Embryos
manufacturer’s indications for expiration date. Store at 4°C.Stock 2: Na pyruvate. Dissolve 0.220 g sodium pyruvate in 100 ml water. Sterile-filter into an aluminum-foil wrapped 100 ml bottle and store at 4°C for 1 mo.Stock 3: Bovine Steer Serum (BSS
Basic Methods of Culturing Drosophila
). A convenient method is to transfer eggs to a bleach soaked wedge of filter paper, wick away bleach after chorions have dissolved (3-5 minutes), wash eggs several times with water and transfer to a fresh piece of filter paper (small enough to sit on the surface
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