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文献和实验Sample preparation (analytical gels)
(cerebrospinal fluid): An aliquot of 250 μl of human CSF was mixed with 500 μl of ice cold acetone and centrifuged at 10000 g at 4o C for 10 minutes. The pellet was mixed with 10 μl of a solution containing SDS (10% w/v) and DTE (2.3% w/v). The sample was heated
Sample Preparation for Microinjection
Cultivation and Microinjection Preparation [3] Plate 250 cells in 5 µl droplets in the center of a glass coverslip (10 x 10 mm). Place coverslips into a humid chamber and incubate at 37°C until cells attach to the glass
Sample Preparation for Microinjection
of microinjected cells. Between 500 and 1000 labeled cells are sufficient to analyze labeled proteins by gel electrophoresis. Cultivation and Microinjection Preparation [3] Plate 250 cells in 5 µl droplets in the center of a glass coverslip (10 x
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