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文献和实验Immunohistochemistry: Mouse Anti-Human MCP-1
. 4. Incubate the tissue section overnight at 4?C with Mouse Anti-Human MCP-1 at 10.0 μg/mL. Wash the slide twice for three minutes each. 5. Incubate the tissue section with the Envision HRP polymer kit (Dako) according to the manufacturer’s
and you can slide the pick out from under the worm so that the worm lies relatively flat and extended Inject worm set up injector: turn on micro-injector open valve on pressure tank open valve of pressure regulator
Whole-mount in situ hybridization for the detection of RNA in C. elegans embryos
Mounting media: 70% glycerol, 1mg/ml p-phenylenediamine (pH 9). C. Working solutions These solutions are prepared on the day of use.- Formaldehyde fixative solution: 1X PBS, 0.08 M Hepes (pH 6.9), 1.6 mM MgSO4, 0.8 mM EGTA, 3.7% formaldehyde.-
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