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文献和实验Patch-clamp is the best method for analyzing single-channel current activities today. The method is not good, however, at measuring the total number of channels expressed on the cell membrane that are responsible for the current of concern
The ability to record the real-time movement of a protein is quite enthralling; in effect, this is what we achieve when we record the activity of an individual ion channel. Clearly we do not “see” the amino acids that make
In this chapter, a detailed protocol is given for ion-channel reconstitution in the two most used model membranes: planar bilayers and liposomes. In the planar bilayer section, methods are described for the expression of ion channels
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