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文献和实验实验试剂 Magnet Mixer allowing both tilting and rotation Buffer: PBS (without Ca2 and Mg2 ) w/0.1% BSA and anticoagulant, pH 7.4 DNase I (when required) Culture Medium: e.g. RPMI 1640 with 2% Fetal
Constructing a Low-budget Laser Axotomy System to Study Axon Regeneration in C. elegans
, set the pulse frequency to 100, continuous mode, and reduce power to a minimum using the half-wave plate. Use a Post-It note or lens paper to visualize the laser beam. 5) Adjust and fix the kinematically mounted mirrors in sequence
MCDB-131, 1X Medium Cellgro 15-100-CV Barbed Polypropylene Fittings Cole Parmer Instrument Co.
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